spp1 growth factor  (PeproTech)

Journal: Bone Research

Article Title: Targeting angiogenesis for fracture nonunion treatment in inflammatory disease

doi: 10.1038/s41413-021-00150-4

Figure Lengend Snippet: SPP1 and CXCL12 restored angiogenesis under inflammation in vitro. a Real-time qPCR analyses were performed to determine the relative expression of Spp1 and Cxcl12 in 10 dpf fracture calluses from control and RA mice ( n = 4). The mRNA levels were normalized to that of Actb and then normalized to the control group. * P < 0.05 compared with control by Student’s t test. b Immunohistochemical staining of 10 dpf fracture calluses from control and RA mice for SPP1 and CXCL12. c Representative images from HUVEC migration and tube formation assays using culture medium from vehicle- and IL-1β-treated chondrocytes supplemented with SPP1 and CXCL12, respectively. Quantification of ( d ) HUVEC migration as well as ( e ) tube number and tube length ( n = 3). All results were normalized to the controls. * P < 0.05 compared with control by two-way ANOVA. Scale bar, 200 μm

Article Snippet: The growth factors 100 μg·mL −1 SPP1 (Peprotec, #120-35) and 20 μg·mL −1 CXCL12 (Peprotec, #300-28B) were mixed with 0.5% gelatin (Millipore Sigma, #G6650) in syringe pump C. PCL was injected at a rate of 5 mL·h −1 onto the collecting mandrel 20 cm away.

Techniques: In Vitro, Expressing, Control, Immunohistochemical staining, Staining, Migration

Journal: Bone Research

Article Title: Targeting angiogenesis for fracture nonunion treatment in inflammatory disease

doi: 10.1038/s41413-021-00150-4

Figure Lengend Snippet: PCL scaffolds gradually released SPP1 and CXCL12 in vitro. a Schematic illustration of the simultaneous electrospinning and electrospraying system for PCL scaffold fabrication. b Representative confocal images of the PLGA microspheres encapsulated with growth factors. PLGA: red; growth factor: green. c Representative SEM images of the PCL scaffold. d Profiles of SPP1 and CXCL12 release from PCL scaffolds loaded with SPP1 and CXCL12 ( n = 5). Scale bar, 200 μm

Article Snippet: The growth factors 100 μg·mL −1 SPP1 (Peprotec, #120-35) and 20 μg·mL −1 CXCL12 (Peprotec, #300-28B) were mixed with 0.5% gelatin (Millipore Sigma, #G6650) in syringe pump C. PCL was injected at a rate of 5 mL·h −1 onto the collecting mandrel 20 cm away.

Techniques: In Vitro

Journal: Bone Research

Article Title: Targeting angiogenesis for fracture nonunion treatment in inflammatory disease

doi: 10.1038/s41413-021-00150-4

Figure Lengend Snippet: The release of SPP1 and CXCL12 promoted angiogenesis under inflammatory conditions. a Schematic illustration of the collagen construct that was used to create the 3D cell culture environment to examine the impact of released growth factors on HUVEC migration and tube formation. b Quantification of HUVEC numbers at different depths in collagen gel ( n = 5). All results were normalized to the controls. * P < 0.05 compared with control by two-way ANOVA. c Representative images of HUVEC lumen formation in collagen gels. d Quantification of lumen density at different depths in the collagen gel ( n = 5). * P < 0.05 compared with control by two-way ANOVA. Scale bar, 200 μm

Article Snippet: The growth factors 100 μg·mL −1 SPP1 (Peprotec, #120-35) and 20 μg·mL −1 CXCL12 (Peprotec, #300-28B) were mixed with 0.5% gelatin (Millipore Sigma, #G6650) in syringe pump C. PCL was injected at a rate of 5 mL·h −1 onto the collecting mandrel 20 cm away.

Techniques: Construct, Cell Culture, Migration, Control

Journal: Bone Research

Article Title: Targeting angiogenesis for fracture nonunion treatment in inflammatory disease

doi: 10.1038/s41413-021-00150-4

Figure Lengend Snippet: The controlled release of SPP1 and CXCL12 restored angiogenesis and fracture nonunion in RA mice. a PCL scaffold with or without SPP1 and CXCL12 was applied to the fractured bone in RA mice. b MicroCT assessment of newly formed vessels within fracture calluses from RA mice treated with scaffolds with or without SPP1 and CXCL12 ( n = 5) at 10 dpf. c Quantification of the vessels in RA fracture calluses ( n = 5) at 10 dpf based on microCT assessment. The results were normalized to the scaffold only group. * P < 0.05 compared with control by Student’s t- test. d Immunohistochemical staining for endomucin in fracture calluses from RA mice at 10 dpf. e Quantification of the vessels in RA fracture calluses at 10 dpf ( n = 5) based on the immunohistochemical assessment. The results were normalized to the scaffold only group. * P < 0.05 compared with control by Student’s t test. f Representa t ive ABH/OG staining of fracture callus sections from RA mice treated with scaffold with or without SPP1 and CXCL12 at 10 dpf ( n = 5). g Histomorphometric quantification of bone area was performed on 10 dpf fracture callus sections from RA mice treated with scaffolds with or without SPP1 and CXCL12 ( n = 5). The results were normalized to the scaffold group. h Biomechanical torsion testing of RA fractures treated with scaffold with or without SPP1 and CXCL12 at 28 dpf ( n = 8). Max torque and displacement at max were quantified. All results were normalized to the controls. * P < 0.05 compared with control by Student’s t test. Scale bar, 200 μm

Article Snippet: The growth factors 100 μg·mL −1 SPP1 (Peprotec, #120-35) and 20 μg·mL −1 CXCL12 (Peprotec, #300-28B) were mixed with 0.5% gelatin (Millipore Sigma, #G6650) in syringe pump C. PCL was injected at a rate of 5 mL·h −1 onto the collecting mandrel 20 cm away.

Techniques: Control, Immunohistochemical staining, Staining

Journal: Bone Research

Article Title: Targeting angiogenesis for fracture nonunion treatment in inflammatory disease

doi: 10.1038/s41413-021-00150-4

Figure Lengend Snippet: Primer sequences for qPCR

Article Snippet: The growth factors 100 μg·mL −1 SPP1 (Peprotec, #120-35) and 20 μg·mL −1 CXCL12 (Peprotec, #300-28B) were mixed with 0.5% gelatin (Millipore Sigma, #G6650) in syringe pump C. PCL was injected at a rate of 5 mL·h −1 onto the collecting mandrel 20 cm away.

Techniques: